![]() ![]() oleifera.Ĭamellia oleifera allele dosage genetic differentiation genetic diversity genetic structure polyploid.Īndrew, R. oleifera genetic resources for the breeding of cultivated C. This method and findings can facilitate the utilization of wild C. ![]() oleifera across geographical ranges agree with the "central-marginal hypothesis", stating that genetic diversity is high in the central population and declines from the central to the peripheral populations, and genetic differentiation increases from the centre to the periphery. The genetic variation patterns of wild C. The results demonstrated that the new method could resolve allele dosage uncertainty and considerably improve genetic diversity, structure and differentiation analyses for polyploids. Using 35 SSR markers, the high-throughput sequencing-based SSRSeq method was applied to obtain accurate hexaploid genotypes of wild C. oleifera populations were sampled across geographical ranges in subtropical evergreen broadleaf forests of China. oleifera is essential for the conservation and utilization of genetic resources. Understanding the geographical pattern of genetic variation in wild C. ![]() Hexaploid Camellia oleifera is the dominant woody oilseed crop in China. Perl scripts and an online SSR genotyping tool "SSRSeq" were provided to process the sequencing data and output genotypes with corrected allele dosages. Corrections were performed accounting for the "stutter peak" and amplification efficiency of SSR sequences. The frequency distribution of SSR sequences was obtained based on deep-coverage high-throughput sequencing data. Here, a new SSR genotyping method was developed to directly infer accurate polyploid genotypes. Conventional microsatellite (simple sequence repeat, SSR) genotyping methods cannot accurately identify polyploid genotypes leading to allele dosage uncertainty, introducing biases in population genetic analysis. ![]()
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